Dış Kaynaklı Proje Süreçleri Yönetim Sistemi
Suer İ. (Araştırmacı), Öztürk Ş. , Kaya M. , Abuaısha A.
Breast cancer is the most common cancer among women. It is very important to find new biomarkers for early detection, treatment and prognosis of breast cancer. Circular RNAs (circRNA) are a new class of non-coding RNA that act as microRNA (miRNA) sponges. Although it is known that circRNAs play a role in breast cancer, which has a very heterogeneous structure, studies on the roles of circRNAs in breast cancer subtypes are quite insufficient. In our study, it will be determined whether circRNAs have a role in the determination of breast cancer subtypes and the effect of circRNA-miRNA-Target Gene interaction on breast cancer processes. In the first step of our research, breast cancer tissue samples obtained from 30 luminal A (Lum A), 30 luminal B (Lum B), 20 Triple Negative (TNBC) breast cancer patients within the scope of the doctoral thesis project supported by Istanbul University BAP unit and 40 adjacent normal The expression level of ciRS-7/miR-7-5p and 2 target genes in tissue samples will be investigated. Within the scope of TÜSEB project support, the functional studies of ciRS-7 and miR-7-5p on breast cancer cell lines and the ciRS-7/miR-7-5p/target gene association will be investigated in breast cancer cell lines. In the study to be carried out within the scope of the TÜSEB project, the siRNA of cirRS-7 will be transfected to MCF-10A normal breast epithelial cell, MCF-7 Lum A; BT-474 Lum B; MDA-MB-23 TNBC breast cancer cell lines and the effect of ciRS-7 on cellular processes will be investigated. By the in silico anlaysis, miR-7-5p target genes in breast cancer will be identified and the expression levels of these genes at the mRNA and protein level (by western analysis) will be investigated in cell lines transfected with ciRS-7 siRNA and mimic miR-7-5p. A selected ciRS-7/miR-7-5p/target gene association will then be confirmed by the luciferase reporter assay. In conclusion, with our study, the importance of ciRS-7/miR-7-5p/target gene association in determining breast cancer subtype and the role of circRNA/miRNA/gene interaction in the pathogenesis of breast cancer will be clarified and breast cancer-specific candidate biomarker detection will be possible.